High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects

Kleinstiver et al. (2016). Nature DOI: 10.1038/nature16526 Citations: 2665

Parts used

SpCas9-HF1 Nuclease

Key findings

  • Engineered SpCas9-HF1 with four mutations reducing off-target activity. Maintained on-target efficiency while achieving undetectable off-targets. Established rational design for specificity enhancement.