Cas12a Nuclease

Characteristics

Alternative to Cas9 with staggered overhang cuts instead of blunt ends. Single crRNA (no tracrRNA needed) simplifies system. AT-rich PAM expands targeting options. Can process its own crRNA array for multiplex editing.

Literature References

1. Zetsche et al. (2015). Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system. Cell - Zetsche 2015 Cas12a