Improved LbCas12a variants with altered PAM specificities further broaden the genome targeting range of Cas12a nucleases

Tóth et al. (2020). Nucleic Acids Res DOI: 10.1093/nar/gkaa110 Citations: 157

Key findings

Generated LbCas12a-RVRR variant with TNTN PAM consensus (including TACV, TTCV, CTCV, CCCV). The D156R mutation in impLbCas12a further increased activity in PAM-dependent manner. Expanded targeting range to ~95% of genome.

Parts used

impLbCas12a NucleaseLbCas12a-RVRR Nuclease