Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing
Key findings
Developed enAsCas12a with substantially expanded targeting range, enabling editing at many previously inaccessible PAMs beyond canonical TTTV. Created high-fidelity variant (enAsCas12a-HF1) with reduced off-target effects. Enhanced multiplex gene editing, endogenous gene activation, and C-to-T base editing efficiency.