Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein

Shaner et al. (2004). Nat Biotechnol DOI: 10.1038/nbt1037 Citations: 4615

Parts used

mCherrymOrange2tdTomato

Key findings

  • Engineered mCherry, mOrange, tdTomato, and related variants from DsRed through iterative mutagenesis. mCherry is monomeric, photostable, and fast-maturing with emission at 610 nm. tdTomato is a tandem dimer with the highest brightness of the series. These became the standard red-spectrum reporters for mammalian imaging.