A mouse model for adult cardiac-specific gene deletion with CRISPR/Cas9

Carroll et al. (2016). Proc Natl Acad Sci USA DOI: 10.1073/pnas.1523918113 Citations: 238

Key findings

First cardiac-specific Cas9 transgenic mouse model (Myh6-Cas9). Delivered sgRNA-only AAV9 vectors (U6-sgRNA-CMV-ZsGreen) for postnatal cardiac editing without dual vector complexity. Demonstrated rapid gene deletion in adult cardiomyocytes, circumventing embryonic lethality.

Parts used

SpCas9 Nuclease CMV Promoter AAV9 Serotype U6 Promoter