dCas9-VPR Enhanced CRISPRa Nuclease

Length: 1 bp(0 aa)

Function: Transcriptional Activation

Base protein: dCas9 Catalytically Dead Nuclease

Architecture

Catalytically dead Cas9 fused to tripartite activator combining VP64, p65 activation domain from NF-κB, and Rta from Epstein-Barr virus.

Characteristics

Enhanced CRISPRa system with stronger activation than VP64 alone. Achieves 10-1000× upregulation at many loci. Particularly effective for activating genes with weak endogenous expression. More potent but slightly larger than simple VP64 fusion.

Literature References

  1. Mali et al. (2013). CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering. Nat Biotechnol - Mali 2013 Cas9 Nickase
  2. Chavez et al. (2015). Highly efficient Cas9-mediated transcriptional programming. Nat Methods - Chavez 2015 VPR

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