KRAB Domain

Length: 1 bp

Class: Transcriptional Repressor

Origin: Human KOX1 protein (Krüppel-associated box domain)

Mechanism

Recruits KAP1 (KRAB-associated protein 1) corepressor complex, which in turn recruits histone deacetylases, histone methyltransferases, and heterochromatin protein 1. Creates repressive chromatin state at target loci.

Characteristics

Standard transcriptional repression domain for CRISPRi. Strong repression (80-99% knockdown typical). Creates stable heterochromatin marks. Repression reversible but can persist for weeks. Compact size (~75 amino acids). More specific than RNAi.

Literature References

  1. Mali et al. (2013). CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering. Nat Biotechnol - Mali 2013 Cas9 Nickase

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