xCas9 Nuclease

Length: 4100 bp(1366 aa)

PAM: NG, GAA, GAT

Origin: Streptococcus pyogenes (engineered)

Characteristics

Expanded PAM recognition broadens targeting range. Recognizes NG, GAA, and GAT PAMs versus wild-type NGG. Maintains SpCas9 efficiency with 4x more target sites available. Requires dual-AAV system for in vivo delivery.

Literature References

  1. Hu et al. (2018). Evolved Cas9 variants with broad PAM compatibility and high DNA specificity. Nature - Hu 2018 xCas9

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