Evolved Cas9 variants with broad PAM compatibility and high DNA specificity

Hu et al. (2018). Nature DOI: 10.1038/nature26155 Citations: 1647

Parts used

xCas9 Nuclease

Key findings

  • Evolved xCas9 with expanded PAM recognition (NG, GAA, GAT). Increased targetable sites 4-fold while maintaining high specificity. Demonstrated phage-assisted continuous evolution methodology.