SaCas9 Nuclease

Compact Staphylococcus aureus Cas9 nuclease (3.2 kb) that fits alongside a guide RNA and regulatory elements within a single AAV vector. Requires NNGRRT PAM, with the KKH variant expanding recognition to NNNRRT for broader genomic coverage. First validated compact alternative to SpCas9 for in vivo gene editing in size-constrained therapeutic vectors.

Length: 3200 bp(1066 aa)

PAM: NNGRRT

Origin: Staphylococcus aureus

Characteristics

Compact size enables single-AAV packaging with guide RNA and regulatory elements. First alternative to SpCas9 for in vivo applications. More restrictive PAM limits targeting flexibility. KKH variant improves PAM recognition to NNNRRT.

Literature References

  1. Ran et al. (2015). In vivo genome editing using Staphylococcus aureus Cas9. Nature - Ran 2015 SaCas9

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