Engineered CRISPR-Cas9 nucleases with altered PAM specificities

Kleinstiver et al. (2015). Nat Biotechnol DOI: 10.1038/nbt.3404 Citations: 644

Parts used

SaCas9-KKH Nuclease

Key findings

  • Developed KKH-SaCas9 variant with relaxed PAM (NNNRRT vs NNGRRT). Expanded targetable sites by ~2-fold while maintaining efficiency and specificity.