dCas9-KRAB CRISPRi Nuclease

Length: 1 bp(0 aa)

Function: Transcriptional Repression

Base protein: dCas9 Catalytically Dead Nuclease

Architecture

Catalytically dead Cas9 fused to KRAB (Krüppel-associated box) repressor domain from human KOX1 protein. KRAB recruits histone deacetylases and heterochromatin-forming complexes.

Characteristics

Standard CRISPRi system for gene knockdown without DNA cleavage. Typically achieves 80-99% reduction in target gene expression. More specific than RNAi with minimal off-target effects. Repression is reversible and can be maintained for weeks in culture.

Literature References

  1. Mali et al. (2013). CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering. Nat Biotechnol - Mali 2013 Cas9 Nickase

Related collections

1 4