dCas9-KRAB CRISPRi Nuclease

Length: 1 bp(0 aa)

Function: Transcriptional Repression

Base protein: dCas9 Catalytically Dead Nuclease

Characteristics

Standard CRISPRi system for gene knockdown without DNA cleavage. Typically achieves 80-99% reduction in target gene expression. More specific than RNAi with minimal off-target effects. Repression is reversible and can be maintained for weeks in culture.

Architecture: Catalytically dead Cas9 fused to KRAB (Krüppel-associated box) repressor domain from human KOX1 protein. KRAB recruits histone deacetylases and heterochromatin-forming complexes.

References

  1. Mali et al. (2013). CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering. Nat Biotechnol - Mali 2013 Cas9 Nickase

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