pCMV-GFP
Mammalian expression plasmid in which a CMV enhancer and promoter drives EGFP, followed by a rabbit β-globin polyadenylation signal. Deposited by the Cepko lab as Addgene #11153 on a pCAGEN backbone; Matsuda and Cepko made it by replacing the CAG promoter of pCAG-GFP with the CMV promoter from pEGFP-N1.
Characteristics
4,479 bp circular plasmid with ampicillin resistance in bacteria. In the developing rat retina, GFP from the CMV promoter was lower than from the EF1α or ubiquitin C promoters, and it labeled cells with bodies in the inner nuclear layer more than photoreceptors, suggesting weak activity in photoreceptors.
Parts
References
- Matsuda et al. (2003). Electroporation and RNA interference in the rodent retina in vivo and in vitro. Proceedings of the National Academy of Sciences- Matsuda 2003 Retinal Electroporation
Available products
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Additional information
Applications: Expressing GFP from the CMV promoter in mammalian cells; in the retina work that introduced it, CMV was a poor choice when photoreceptors need to be labeled.