Rabbit β-Globin (rBG) PolyA Signal

Natural polyadenylation signal from the rabbit β-globin gene (56 bp) with ~100% transcription termination efficiency and 1.4–2.5-fold increase in polyadenylated RNA over baseline. Ultra-compact size makes it suitable for size-critical AAV vectors, and it serves as the evolutionary template for the synthetic SPA signal. High efficiency with minimal sequence footprint.

Length: 56 bp

Efficiency: Very High

Transcript stability: Exceptionally efficient termination and polyadenylation resulting in stable, properly processed mRNA

Origin: Rabbit β-globin gene 3' untranslated region

Characteristics

Natural highly efficient polyA signal containing all elements required for efficient cleavage and polyadenylation. Signals >90% of polyadenylation events when tested. Increases fraction of polyadenylated viral RNAs by 1.4-2.5 fold. Nearly 100% transcription termination efficiency. Contains position-dependent downstream elements required for efficient 3' end formation. Serves as template for synthetic polyA optimization.

Applications: Applications requiring maximum polyadenylation and termination efficiency. Template for designing minimal synthetic polyA signals. Transgenic animals and stable cell line generation. Used when transcription termination efficiency is critical parameter.

Limitations: Moderate size (~94 bp) larger than synthetic but smaller than SV40/bGH. Less commonly available in commercial vectors. Position-dependent efficiency may require optimization in novel contexts.

Sequence

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References

  1. Lanoix & Acheson (1988). A rabbit beta-globin polyadenylation signal directs efficient termination of transcription of polyomavirus DNA. EMBO J - Lanoix 1988 Rabbit Beta-Globin