Ultra-compact CRISPR system for payload-limited applications. CasMINI (Cas12f1 variant, 529aa) is the smallest known RNA-guided nuclease, enabling maximum cargo capacity in single AAV vectors. Staggered double-strand breaks reduce off-target activity compared to blunt cutters. Compatible with standard AAV serotypes for in vivo delivery. Ideal when vector space is critical or when co-packaging additional elements1,2.
