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CasMINI All-in-One AAV Editor

CasMINI All-in-One AAV Editor

AAV-CASMINI-CMV-XS

$1,995
$1,995
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Promoter
Scale

Ultra-compact CRISPR system for payload-limited applications. CasMINI (Cas12f1 variant, 529aa) is the smallest known RNA-guided nuclease, enabling maximum cargo capacity in single AAV vectors. Staggered double-strand breaks reduce off-target activity compared to blunt cutters. Compatible with standard AAV serotypes for in vivo delivery. Ideal when vector space is critical or when co-packaging additional elements1,2.

 

Specifications

Single stranded AAV produced in 293T cells and purified through two (2) rounds of CsCl ultracentrifugation and stored in 0.001% F-68 in DPBS with additional 150 mM NaCl. Ships on dry ice overnight.

Deliverables

  • Titer: 1e13 VG/mL or higher
  • Full Capsid Ratio: 0.85 or higher
  • Delivery Timeline: 18–21 days

Available Serotypes

This construct can be packaged in hundreds of AAV serotypes to target your specific tissue. We offer all standard serotypes (AAV1-9) plus specialized variants for muscle (MyoAAV-2A, MyoAAV-1A, AAVMyo), brain (PHP.eB, AAV9P31, AAV2-retro), and clinical applications (AAV2, Spark100). Other popular options include AAV-rh.74, AAV6, AAV6.2FF, and AAV3. Browse our complete serotype library or contact us for recommendations.

Parts

  • CMV Promoter: Strong, ubiquitous expression across most mammalian cell types. Provides highest initial transgene levels but may experience silencing in some contexts over time.
  • EFS Promoter: Compact promoter derived from human elongation factor 1-alpha. Provides stable, constitutive expression with reduced silencing compared to viral promoters like CMV.
  • hSyn Promoter: Neuron-specific promoter restricting expression to neurons. Minimal activity in glial cells. Compact size suitable for AAV packaging.
  • hPGK Promoter: Moderate, stable expression with lower immunogenicity than viral promoters. Maintains consistent levels over time without significant silencing.
  • CasMINI Nuclease: Ultracompact nuclease at 40% the size of SpCas9. Engineered from archaeal Cas12f for mammalian cell activity. High specificity with low off-target effects. Staggered cuts reduce large deletions. Leaves significant space for additional genetic elements in AAV.
  • U6 Promoter: RNA Pol III promoter for expressing short RNAs like guide RNAs, shRNAs, and other small non-coding RNAs. Not for protein-coding genes.

References

  1. Efficient CRISPR editing with a hypercompact Cas12f1 and engineered guide RNAs delivered by adeno-associated virus, Kim et al. (2022) Nat Biotechnol 40:94-102
  2. Comprehensive assessment of miniature CRISPR-Cas12f nucleases for gene disruption, Xin et al. (2022) Nat Commun 13:5623
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