Promoter of the zebrafish (Danio rerio) ubiquitin (ubi) gene, comprising the 5' flanking region plus the first noncoding exon and intron. Drives strong, ubiquitous transgene expression from the mid-blastula transition through adulthood across essentially all examined tissues, and is a standard constitutive driver for zebrafish transgenesis and reporter lines.
Length: 3483 bp
Strength: strong
Tissue: Ubiquitous — all examined embryonic and adult tissues in zebrafish, including blood cells, retina, and fin (Mosimann et al. 2011).
Expression: constitutive
Origin: Danio rerio (zebrafish) ubi gene
Characteristics
The ~3.5 kb ubi promoter fragment, including the first noncoding exon and intron of the zebrafish ubiquitin gene, drives constitutive transgene expression from the mid-blastula transition onward, sustained through adulthood in all analyzed organs, including erythrocytes and fin, tissues where beta-actin and ef1a promoter fragments show weak or progressively silenced activity (Mosimann et al. 2011). It was used to build the Cre-responsive ubi:loxP-EGFP-loxP-mCherry switch reporter and the ubi:CreERT2 tamoxifen-inducible driver line; by flow cytometry it gives robust reporter expression across all resolvable blood cell lineages, and transplanted hematopoietic cells retain reporter expression and multilineage engraftment for at least three months (Mosimann et al. 2011).
Applications: Used in Tol2-transposon-based zebrafish transgenesis for ubiquitous reporter lines, Cre/lox lineage-tracing and switch constructs (ubi:Switch), and tamoxifen-inducible CreERT2 driver lines; suited to marking transplanted hematopoietic cells and tracking multilineage engraftment in contexts where beta-actin or ef1a promoters lose activity (Mosimann et al. 2011).
Limitations: As with other Tol2-delivered transgenes, expression level and consistency depend on genomic integration site and copy number, so independent transgenic lines require individual validation. Comprehensive characterization is currently limited to the founder lines and assays reported by Mosimann et al. (2011).
Mosimann et al. (2010).
Ubiquitous transgene expression and Cre-based recombination driven by the ubiquitin promoter in zebrafish.
Development - Mosimann 2010 Zebrafish ubi Promoter
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