Tetracycline-responsive element (TRE) 2nd-generation promoter

Tetracycline response element (TRE) promoter containing seven Tet operator sequences upstream of a minimal CMV promoter; active only when bound by the rtTA (Tet-On) or tTA (Tet-Off) transactivator. Core component of doxycycline-inducible expression systems.

Length: 424 bp

Characteristics

Synthetic inducible; 7× tetO copies upstream of minimal CMV promoter. Activated by tTA (Tet-Off) or rtTA (Tet-On). Gossen & Bujard, 1992; some background activation without transactivator.

Sequence

ggtaccgagctcgactttcacttttctctatcactgatagggagtggtaaactcgactttcacttttctctatcactgatagggagtggtaaactcgactttcacttttctctatcactgatagggagtggtaaactcgactttcacttttctctatcactgatagggagtggtaaactcgactttcacttttctctatcactgatagggagtggtaaactcgactttcacttttctctatcactgatagggagtggtaaactcgactttcacttttctctatcactgatagggagtggtaaactcgacctatataagcagagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagacaccgggaccgatccagcctccgcggccccgaattg

Literature References

  1. Gossen et al. (1992). Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc Natl Acad Sci USA - Gossen 1992 Tet-Off
  2. Gossen et al. (1995). Transcriptional activation by tetracyclines in mammalian cells. Science - Gossen 1995 Tet-On