Library › Parts › Promoters › PrP Mouse prion protein (Prnp) promoter (Borchelt 'MoPrP.Xho' half-genomic vector) Mouse prion protein (Prnp) gene promoter, including its 5' flanking region, untranslated first exon, first intron, and part of exon 2, packaged as the "MoPrP.Xho" expression vector. Drives broad, high-level expression across CNS neurons and in cardiac muscle, closely mirroring endogenous PrP except that it does not direct expression in cerebellar Purkinje cells. Widely used to build transgenic mouse models of neurodegenerative disease.
Origin: Mus musculus Prnp gene (Borchelt MoPrP.Xho half-genomic vector)
Characteristics Comprises Prnp 5' flanking sequence, the untranslated first exon, first intron, and part of exon 2, followed by an untranslated 3' region from the endogenous gene, with foreign coding sequence inserted at a unique XhoI site between exon 2 and the 3' UTR (Borchelt et al. 1996). Drives high-level transgene expression broadly across CNS neurons and in cardiac muscle, reproducing the developmental and spatial pattern of endogenous PrP with one documented exception — it does not direct expression in cerebellar Purkinje cells (Borchelt et al. 1996).
Applications: Standard backbone for generating transgenic mouse models of neurodegenerative disease, including tauopathy and synucleinopathy models; used, for example, to drive CNS neuron-wide expression of mutant human A53T alpha-synuclein in a mouse model of Lewy body pathology and movement disorder (Giasson et al. 2002).
Limitations: Does not express in cerebellar Purkinje cells despite otherwise broad CNS neuronal activity, ruling it out when Purkinje-cell-inclusive expression is required (Borchelt et al. 1996). As a pronuclear-injection transgene rather than a targeted knock-in, expression level and onset can vary with transgene copy number and chromosomal integration site.
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