SaCas9 Nuclease

Characteristics

Compact size enables single-AAV packaging with guide RNA and regulatory elements. First alternative to SpCas9 for in vivo applications. More restrictive PAM limits targeting flexibility. KKH variant improves PAM recognition to NNNRRT.

Literature References

1. Ran et al. (2015). In vivo genome editing using Staphylococcus aureus Cas9. Nature - Ran 2015 SaCas9