NmeCas9 Nuclease

Length: 3200 bp(1066 aa)

PAM: NNNNCC

Origin: Neisseria meningitidis

Characteristics

Compact size for single-AAV delivery. GC-rich PAM complements AT-rich PAMs of Cas12 variants. Lower seroprevalence than SaCas9 in human populations. Efficient editing in cell lines and primary cells.

Literature References

  1. Esvelt et al. (2013). Orthogonal Cas9 proteins for RNA-guided gene regulation and editing. Nat Methods - Esvelt 2013 NmeCas9

Related collections

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