hfCas12Max Nuclease

Length: 3200 bp(1066 aa)

PAM: TN or TTN

Origin: Cas12i (engineered via directed evolution)

Characteristics

Broad PAM recognition enables targeting of AT-rich regions. High-fidelity editing with minimal off-targets. Size allows single-AAV packaging. Efficient in primary cells and iPSCs. Balance of compactness and targeting flexibility.

Literature References

  1. Zhang et al. (2023). An engineered xCas12i with high activity, high specificity, and broad PAM range. Protein Cell - Zhang 2023 hfCas12MAX

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