CasMINI Nuclease

Length: 1600 bp(533 aa)

PAM: TTTV

Origin: Uncultivated archaea (Un1Cas12f1)

Characteristics

Ultracompact nuclease at 40% the size of SpCas9. Engineered from archaeal Cas12f for mammalian cell activity. High specificity with low off-target effects. Staggered cuts reduce large deletions. Leaves significant space for additional genetic elements in AAV.

Literature References

  1. Xu et al. (2021). Engineered miniature CRISPR-Cas system for mammalian genome regulation and editing. Mol Cell - Xu 2021 CasMINI
  2. Karvelis et al. (2020). Transposon-associated TnpB is a programmable RNA-guided DNA endonuclease. Nature - Karvelis 2020 Cas12f

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