Human c-Myc Nuclear Localization Signal (cMyc NLS)

Compact 9-residue nuclear localization signal (PAAKRVKLD) from human c-Myc with >85% nuclear targeting efficiency in mammalian cells. Doubles as a Myc epitope tag detectable by the 9E10 antibody, enabling simultaneous nuclear localization and immunodetection with a single sequence. Functional alternative to SV40 NLS used in base editing and gene editing constructs where Myc-tag detection is also required.

Length: 27 bp

Subtype: Nuclear localization signal

Target: Nucleus

Efficiency: >85% nuclear in mammalian cells

Origin: Human c-Myc proto-oncogene

Characteristics

Basic NLS (PAAKRVKLD). Importin-α–dependent pathway, similar to SV40 NLS. Slightly lower intrinsic efficiency than SV40 NLS but fully functional. Dual utility: the 9E10 antibody recognizes the Myc tag within this sequence.

Applications: Alternative NLS when Myc-tag detection is also needed. Stacking with SV40 NLS in dual-NLS constructs for improved nuclear targeting. Used in some gene editing and base editing systems.

Limitations: Slightly weaker than SV40 NLS in direct comparisons. The 9E10 antibody recognizes this region—if antibody staining is used for other purposes, background considerations apply. Constitutively active like SV40 NLS.

Sequence

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References

Evan et al. (1985). Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol Cell Biol - Evan 1985 cMyc NLS