Generation and characterization of chimeric recombinant AAV vectors

Hauck et al. (2020). Mol Ther Methods Clin Dev DOI: 10.1016/s1525-0016(03)00012-1 Citations: 230

Key findings

AAV-ShH10Y445F combines ShH10 capsid with Y445F tyrosine mutation. Enhanced intracellular trafficking through reduced ubiquitination. 2-5 fold improved transduction versus wild-type ShH10 in liver and muscle. Applied in hemophilia B clinical development.