The 3'-flanking sequence of the bovine growth hormone gene contains novel elements required for efficient and accurate polyadenylation
Key findings
Characterized bGH 3'-flanking sequence requirements showing that bGH polyA signal contains a diffuse efficiency element rather than discrete GU- or U-rich motifs found in other signals. Deletion and block mutation analysis revealed that bGH polyadenylation efficiency depends on distributed sequence elements, establishing molecular basis for its unique properties and widespread use in expression vectors.