Determined the complete nucleotide sequence of the human growth hormone (hGH) gene, including the 3' flanking region containing the polyadenylation signal (AATAAA hexamer + downstream elements). This sequence defined the hGHpA region that was subsequently extracted as a ~250 bp cassette and incorporated into mammalian and AAV expression vectors. Not an expression vector paper—the polyA use is a downstream application of the sequence they published.
Human growth hormone DNA sequence and mRNA structure: possible alternative splicing
Parts used
Key findings
Abstract
We have determined the complete sequence of the human growth hormone (hGH) gene and the position of the mature 5' end of the hGH mRNA within the sequence. Comparison of this sequence with that of a cloned hGH cDNA shows that the gene is interrupted by four intervening sequences. S1 mapping shows that one of these intervening sequences has two different 3' splice sites. These alternate splicing pathways generate hGH peptides of different sizes which are found in normal pituitaries. Comparison of sequences near the 5' end of the hGH mRNA with a similar region of the alpha subunit of the human glycoprotein hormones reveals an unexpected region of homology between these otherwise unrelated peptide hormones.