AAV-PHP.eB Serotype

Second-generation CNS-tropic AAV9 variant delivering 40-fold greater brain transduction than AAV-PHP.B via LY6A receptor after systemic injection in C57BL/6 mice. Achieves widespread coverage of neurons, astrocytes, and oligodendrocytes across brain and spinal cord. Most commonly used PHP series variant for whole-CNS gene delivery in neuroscience research.

Length: 1 bp

Receptor: LY6A (Ly6a in mice)

Tropism: CNS (neurons, astrocytes, oligodendrocytes), PNS (mouse-specific)

Characteristics

Second-generation CREATE evolution from PHP.B. Enhanced transduction efficiency across CNS cell types. Broader neuronal and glial tropism. LY6A receptor-dependent. Superior performance versus PHP.B series in C57BL/6 mice.

Applications

Mouse CNS gene therapy. Widespread brain and spinal cord transduction. Neuroscience research tool (most commonly used PHP variant). Cell-type specific promoter studies.

Literature References

  1. Chan et al. (2017). Engineered AAVs for efficient noninvasive gene delivery to the central nervous system. Nat Neurosci - Chan 2017 PHP.eB
  2. Hordeaux et al. (2018). The GPI-linked protein LY6A drives AAV-PHP.B transport across the blood-brain barrier. Mol Ther - Hordeaux 2018 LY6A

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