Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity
Key Finding
Fourth-generation base editor (BE4) with dual UGI domains and extended linkers reduced indels 2.3-fold and non-C-to-T byproducts 2.3-fold versus BE3. Gam protein fusion further decreased unwanted editing products.