Enhanced prime editing systems by manipulating cellular determinants of editing outcomes

Chen et al. (2021). Cell DOI: 10.1016/j.cell.2021.09.018 Citations: 811

Key findings

Developed PE4 and PE5 variants with MLH1dn fusion to evade mismatch repair. Achieved 2-6 fold efficiency improvement. Established cellular pathway manipulation for editing enhancement.

Parts used

PE4 Prime EditorPE5 Prime Editor