Search-and-replace genome editing without double-strand breaks or donor DNA

Anzalone et al. (2019). Nature DOI: 10.1038/s41586-019-1711-4 Citations: 5334

Key findings

Developed prime editing enabling all 12 base-to-base conversions plus insertions/deletions without DSBs or donor DNA. Fused reverse transcriptase to Cas9 nickase with prime editing guide RNA (pegRNA).

Parts used

PE2 Prime Editor